Document 1884
 DOCN  M94A1884
 TI    Molecular mimicry between V3 loop of HIV-1 gp120 and endogenous human
       proteins.
 DT    9412
 AU    Trujillo JR; McLane MF; Lee TH; Essex M; Harvard School of Public
       Health, Cancer Bio. Dept., Boston, MA.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):43 (abstract no. 144A). Unique
       Identifier : AIDSLINE ICA10/94370691
 AB    OBJECTIVE: Viral infections can induce autoimmune responses by several
       mechanisms including molecular mimicry. To determine the role of the
       cellular immune response in HIV pathogenesis, antibodies to gp120, a
       highly immunogenic protein of HIV-1, were analyzed for cross-reactivity
       with endogenous human proteins. METHODS AND RESULTS: Homogenized brain,
       kidney, liver, lung and spleen tissues from HIV-1 seronegative
       individuals as well as lysates from various cell lines were fractionated
       by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
       Initial western blot analyses of human brain proteins (HBP) with several
       monoclonal and polyclonal anti-HIV-1 antibodies including anti-HIV-1
       gp120, anti-gp120 V3 loop (V3mAb), anti-gp120 V5 loop, anti-gp41,
       anti-p24, anti-p17, anti-Vpu and anti-Vpr revealed cross-reactivity only
       with the V3mAb. The V3mAb specifically reacted with three prominent
       human brain proteins of approximately 35, 55 and 110 kDa and with minor
       proteins of 23 and 65 kDa. The 55 kDa human brain protein appears to be
       specific to the central nervous system. Western blot analyses of
       proteins from peripheral tissues and various cell lines were performed
       using the V3mAb and revealed reactivity to 35 and 110 kDa proteins.
       Binding of the V3mAb to endogenous human proteins appears to be specific
       since it was competitively blocked by recombinant HIV-1 gp120.
       DISCUSSION AND CONCLUSIONS: The V3 loop of HIV-1 gp120 appears to share
       an epitope with endogenous human proteins. An immune response to the V3
       loop that can generate cross-reactive antibodies to cellular proteins
       may be an autoimmune mechanism by which HIV-1 can damage cells within
       the central nervous system and other organs.
 DE    Antibodies, Monoclonal  Antigenic Determinants/IMMUNOLOGY/ISOLATION &
       PURIF  Autoantibodies/BIOSYNTHESIS  Autoantigens/ISOLATION & PURIF
       Binding, Competitive  Brain/IMMUNOLOGY  Cell Line  Cross Reactions
       Human  HIV Antibodies  HIV Antigens  HIV Envelope Protein
       gp120/*IMMUNOLOGY  HIV Infections/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       Immunity, Cellular  Molecular Weight  Nerve Tissue
       Proteins/CHEMISTRY/IMMUNOLOGY  Peptide Fragments/*IMMUNOLOGY
       Proteins/*IMMUNOLOGY/ISOLATION & PURIF  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).